Blt Mouse Model

The blt model has a complete and totally functional human immune system with hla restrictedt lymphocytes.

Blt mouse model. These findings show that blt immune humanized mice can demonstrate immune mediated adverse effects of antipd1 therapy and may represent a model that can be used to better understand toxicity of this class of drugs. The blt model is created via surgical transplantation of human fetal liver and thymus under the mouse kidney capsule accompanied with intravenous injection of autologous fetal liver hematopoietic stem cells hscs derived from the same fetal liver donor. The results indicate that the tko blt mouse is a viable model in which to study hiv 1 cure strategies. Although bone marrow liver thymus blt humanized mice provide a robust model for hiv 1 infection and enable evaluation of cure strategies dependent on endogenous immune responses most mice develop graft versus host disease gvhd limiting their utility for extended hiv cure studies.

This hu blt mouse not only will be a useful model for studying the pathogenesis of kshv in vivo but can potentially be used to study the routes and spread of viral infection in the infected host. Checkpoint inhibitor immune humanized mice immunotoxicity pharmaceuticals histopathology toxicity chronic. Following the time to establish an hiv 1 reservoir and achieve virus suppression by art the mice survive at least another five months in a healthy state sufficient time to rigorously test cure strategies. Although bone marrow liver thymus blt humanized mice provide a robust model for hiv 1 infection and enable evaluation of cure strategies dependent on endogenous immune responses most mice develop graft versus host disease gvhd limiting their utility for extended hiv cure studies.

Another humanized mouse model frequently employed in hivaids research is the blt bone marrowliverthymus model. Humanized blt scid mouse models are established by co implanting human fetal liver and autologous thymus fragments under the renal capsule of an immunodeficient mouse and then either before or after preconditioning human hscs isolated from the same fetal liver are injected intravenously to engraft the bone marrow. Ab lack of an effective small animal model to study the kaposis sarcoma associated herpesvirus kshv infection in vivo has hampered studies on the pathogenesis and transmission of kshv. Recently a new generation of humanized mouse the blt bone marrow liver and thymus mouse hu blt generated from nodscidil2rg nsg mouse has been shown to be an excellent model for studying human viral infections 9.